Analysis Description

Paternity/maternity tests are performed where it is necessary to ascertain the child’s biological origins.

 

The analysis is based on the truth that each person inherits a half of one’s DNA molecules (chromosomes) from the mother and a half – from the father. In order to determine the child’s biological origins, the potential father’s, the child’s and the mother’s DNA molecule profiles are compared. DNA profile (STR genotype) of each person is almost unique, because the probability that the two men who are not blood relatives will have identical DNA profile is minimal. This DNA profile characteristics used in paternity test to determine the child’s biological origins.

 

Paternity test accuracy is directly proportional to the number of tested DNA regions (loci). In the recent past in Latvia, DNA profiles with 10 DNA loci were compared in paternity tests. Today, the best method of proving paternity is recognised the DNA profile, in which at least 15 DNA loci are compared in a man’s, child’s and mother’s samples. The loci that are to be analysed are established by international forensic institutions, so today, actually all laboratories test the same set of loci and the results are compatible.

 

A GENUS LLC offers determination of paternity with the system AmpFlSTR® Identifiler Plus™ developed by the company “Applied Biosystems” – recognised world leader in DNA analyses. This system makes it possible to analyse 15 DNA loci.

 

The method is registered and certified by the Health Statistics and Medical Technology Agency, Code La/MoB 068.

 

Interpretation of Results

 

What does a negative result mean?

One can talk about paternity exclusion when the child’s DNA profile in at least two or more DNA loci shows results, which do not coincide with the potential father’s results. In this case, there is no doubt that the tested man is NOT the child’s biological father – a negative result reliability is always 100%.

 

An example of a negative result:

DNS regions

(loci)

Potential father’s

DNA profile

Child’s

DNA profile

Child’s mother’s

DNA profile

Kinship likelihood

D8S1179

10

15

12

15

12

16

is possible

D21S11

26

30

30

32

30

32

is possible

D7S820

10

11

10

12

10

10

is not possible

CSF1PO

9

12

12

12

12

12

is possible

D3S1358

15

15

16

17

15

16

is not possible

TH01

9,3

9,3

7

9,3

6

9,3

is not possible

D13S317

8

12

8

11

11

11

is possible

D16S539

11

11

11

13

12

13

is possible

D2S1338

23

24

24

25

22

24

is not possible

D19S433

14

15

14

15

13

15

is possible

vWA

14

15

15

19

19

20

is possible

TPOX

8

8

8

8

8

11

is possible

D18S51

13

18

13

16

14

16

is possible

Amel

x

y

x

y

x

x

D5S818

12

12

11

12

12

14

is not possible

FGA

23

22.2

23

22

21

22

is possible

Conclusions:

  1. The person from whom the CHILD’S sample is taken is a male.

  2. Likelihood ratio = 0;

  3. Possibility that the TESTED MAN could be the CHILD’S biological father is excluded.

What does a positive result mean?

One can talk about paternity approval when one of the child’s results in all tested DNA loci coincides with the potential father’s results, but the other – with the mother’s the results. It is important to understand that a positive test result always has a very high statistical probability (usually from 99.99 up to more than 99.9999999999%) and not 100%, because there is a possibility that there are other men in the world who have the same result in the tested DNA loci, as the analysed person.

An example of a positive result:

DNS regions

(loci)

Potential father’s

DNA profile

Child’s

DNA profile

Child’s mother’s

DNA profile

Kinship likelihood

D8S1179

10

15

12

15

12

16

is possible

D21S11

26

30

30

32

30

32

is possible

D7S820

10

12

10

12

10

10

is possible

CSF1PO

9

12

12

12

12

12

is possible

D3S1358

15

15

15

16

15

16

is possible

TH01

9,3

9,3

9,3

9,3

6

9,3

is possible

D13S317

8

12

8

11

11

11

is possible

D16S539

11

11

11

13

12

13

is possible

D2S1338

23

25

24

25

22

24

is possible

D19S433

14

15

14

15

13

15

is possible

vWA

14

15

15

19

19

20

is possible

TPOX

8

8

8

8

8

11

is possible

D18S51

13

18

13

16

14

16

is possible

Amel

x

y

x

y

x

x

D5S818

11

12

11

12

12

14

is possible

FGA

23

22.2

23

22

21

22

is possible

Conclusions:

  1. The person from whom the CHILD’S sample is taken is a male.

  2. Likelihood ratio = 10634969;

  3. Possibility that the TESTED MAN could be the CHILD’S biological father is 99,999991%.

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